Antimicrobial activities of naphthazarins from arnebia euchroma. Effect of light quality on regeneration and naphthoquinones. Arnebia euchroma rolye johnst boraginaceae and malva sylvestris l. The plant list includes 69 scientific plant names of species rank for the. The purpose of this study was to evaluate the healing effects of ae ointment aeo on patients with a seconddegree burn wound and compare its results with silver sulfadiazine cream ssd.
Arnebiaeuchroma royle johnst ruanzicao is a traditional chinese herbal medicine tcm. Farjam3 1department of nursing, ahvaz branch, islamic azad university, ahvaz, iran 2research institute for infectious diseases of digestive system and dept of virology, the school of medicine. Natural dyeing of wool with arnebia euchroma optimized by plasma treatment and response surface methodology aminoddin haji 1, zahrasadat amiri 2, sayyed sadroddin qavamnia 1 1textile engineering department, birjand branch, islamic azad university, birjand, iran 2carpet department, faculty of art, university of birjand, birjand, iran. Study on the antio xidant a ctivities of crude extracts. Burn injuries can cause detrimental longterm consequences and call for immediate management. However, because of overexploitation and the fast progress of the industrial activities close to the natural habitat of these herbs, both species are now seriously endangered. The callus was powdered and subjected to extraction by radicalfree tetrahydrofuran thf 11. Pigments 28 are isolated for the first time from arnebia in vitro cultures, 4 and 7 are reported in the present work as novel metabolites within the arnebia genus, while 9 is a known. It is known that hyperactivated complement system involves in the fever and acute lung injury ali in rats. Antimicrobial and antioxidant activity of methanol. Arnebia euchroma ointment was an effective treatment for healing of burn wounds. Fifty rats were divided into 4 equal groups receiving the ointment base, normal saline ns, standard 1% silver sulfadiazine ssd, and 5% and 10% arnebia euchroma ointments aeo. The species is hermaphrodite has both male and female organs and is pollinated by insects. Germination usually takes place within 2 8 weeks at 20c.
When large enough to handle, prick the seedlings out into individual pots and grow them on in the greenhouse for their first winter. Comparison of the percentage of wound contraction between arnebia euchroma and other groups. The proposed method gave the best result with the highest extraction efficiency in the shortest extraction time. Inhibition on platelet activation by shikonin derivatives. The roots are dug in spring or autumn, dried in the sun, soaked in water and cut. Masoud azadbakht, and was kept at the herbarium of faculty of pharmacy, mazandaran university of medical sciences, under the herbarium number. Production of naphthoquinone pigments in cell suspension. Enhancement of shikalkin production in arnebia euchroma callus. Malvaceae are traditionally used to treat various skin disorders. The plant is mainly harvested for its roots which are used in medicines and and as a dye. The healing effect of arnebia euchroma ointment versus silver sulfadiazine on burn wounds in rat ebrahim nasiri 1, seyed jalal hosseinimehr 1, mohammad azadbakht 1, jafar akbari 1, reza enayatifard 2, sohail azizi 3, masoud azadbakht 4 abstract background burn is still a majordevastating condition in emergency. Antioxidantandantimicrobialactivitiesofarnebiahispidissima. In this paper, the effect of ph on in vitro cell growth and naphthoquinone pigment production in cell suspension cultures of arnebia euchroma royle johnston was evaluated under a twostage culture system.
The present study aimed to determine the healing effect of topical treatment with arnebia euchroma on seconddegree burn wound in rats. In our preliminary studies, anticomplementary activity of crude arnebiaeuchroma polysaccharides caep. Deoxyshikonin isolated from arnebia euchroma inhibits. Anionic peroxidase production by arnebia euchroma callus. Arnebia benthamii is one of the promising folklore medicinal plants which is being traditionally used over the years for the treatment of various prevailing diseases in the area. Based on the studies of persian medicine texts, there are some promising bioactivities for this genus that is unknown in modern medicine and some of them are still the basis of new remedies. It is extensively used in china and other countries for treatment of inflammatory diseases. Pdf on may 1, 1993, e roder and others published pyrrolizidine alkaloids from arnebia euchroma find, read and cite all the research you need on researchgate. Molecules free fulltext antimicrobial and cytotoxic. The phytochemical investigation of the nhexane extract from callus and cell suspension culture of arnebia euchroma royle jonst.
The antio xidant activities of aqueous and methanolic e xtracts of the roots of ae and le w ere ev aluated b y t rolo x equiv alent antio xidant capacity tea c assay, dpph staining, free. Isolation and identification of shikonin from arnebia. Enhancement of shikalkin production in arnebia euchromacallus. The cotyledons of sterile seedings were placed on modified ls medium supplemented with 0. The mean of burn area, percentage of wound contraction, histopathological and bacteriological assessments in the injured area were dtermined during the study.
The plant is mainly harvested for its roots which are used in medicines and and as a. Evaluating the effect of metal interference in inhibition of biofilm formation by. The plant list includes a further 10 scientific plant names of infraspecific rank for the genus arnebia. Company and maintained in a specific pathogenfree environment. This whole herb was identified by professor mohammad azadbakht and dr. American journal of advanced drug delivery uk american journal of advanced drug delivery uk original article antioxidant and antimicrobial activities of arnebia hispidissima bharat singh 1 and ram avtar sharma 2 1 institute of biotechnology amity university rajasthan jaipur india. Pdf an efficient and economic method for in vitro propagation of.
Deoxyshikonin isolated from arnebia euchroma inhibits colorectal cancer. Fulltext standardization and antioxidant studies of arnebia hispidissima. The purpose of this study was to evaluate the healing effects of ae ointment aeo on patients with a seconddegree burn. Arnebia euchroma exhibits a potent antihiv activity kashiwada et al. Detailed investigations on the solid cell culture and. Pigments 28 are isolated for the first time from arnebia in vitro cultures, 4 and 7 are reported in the present work as novel metabolites within the arnebia genus, while 9 is a known constituent of both natural roots and in vitro cultures of a. Optimization of shikonin homogenate extraction from arnebia euchroma using response surface methodology. According to previous studies, plasma treatment can improve the dyeability of wool with natural and synthetic dyes haji and shoushtari 2011. Lithospermun root, arnebia root, groomwell root source of earliest record.
This article presents arnebia according to the most important ancient. The genus arnebia family boraginaceae comprises around 25 species. Zhang huizhen 1,2,liao maochuan 2,xuan lijiang 3,guo jixian 1 1. School of pharmacy, fudan university,shanghai 200032,china. Elsevier biochimica et biophysica acta 1268 1995 329334 bb biochi mica et biophysica aeta inhibition on platelet activation by shikonin derivatives isolated from arnebia euchroma fengnien ko a, yisan lee a, shengchu kuo b, yuanshiun chang b, cheming teng a, a pharmacological institute, college of medicine, national taiwan university, taipei, taiwan, roc. Based on singlefactor experiments, a threefactorthreelevel. Friable callus derived from leaves of in vitro established shoots was maintained on ms medium supplemented with bap 10. In our preliminary studies, anticomplementary activity of crude. The roots are dug in spring or autumn, dried in the sun, soaked in water and cut into slices.
The effects of arnebia euchroma ointment on seconddegree. Usda, ars, germplasm resources information network. Catoxantha and megaloxantha are among the few other members of the family that reach a comparable size. Successful indirect regeneration of arnebia pulchra. Animals were allowed free access to laboratory food and water ad libitum.
Contributions from the gray herbarium of harvard university. Pdf the traditional medicine aspects, biological activity. Acetyl shikonin isolated from arnebia euchroma exhibited significant in vivo and in vitro anticancer activity 5. An efficient homogenate extraction technique was employed for extracting shikonin from arnebia euchroma. Kang qiangsheng,li honglin,wu yulan,lu dayan wuhan institute of botany,chinese academy of sciences,wuhan 430074,china. Polysaccharides from arnebia euchroma ameliorated endotoxic. Its flowers are reported to have soothing effect on patients with heart ailments kaul 1997. Arnebia euchroma is a highly valued himalayan plant that has sociocultural and medicinal significance, with almost all parts of the plant being used for medicines, food, dyes and beverages. Pdf on may 1, 1993, e roder and others published pyrrolizidine alkaloids from arnebia euchroma find, read and cite all the research you need.
Naphthoquinone pigments in cell suspension cultures of arnebia euchroma. Determination of the active constituents in arnebia euchroma. Arnebia euchroma herb and roots were bought from the local herbal market in sari, north iran, during september 2014. Study of the antifungal activity of shikonin and alcoholic oily extracts of iranian arnebia euchroma l int j basic sci med. Euchroma gigantea is one of the largest of the buprestid beetles hence the latin name gigantea of the species, reaching a length of about 5080 millimetres 2. The beds are watered frequently and seeds are sown at not more than halfinch depth. Avicennas canon of medicine, describing the use of abukhalsa arnebia euchroma ae as being effective for burn healing. Antimicrobial activities of naphthazarins from arnebia. The root explants of the young plantlets, obtained from the germinated seeds, were used for establishing callus. Antioxidant activity of the plant has been evaluated by the dpph free radical scavenging method. In pursuit of strong shikalkinproducing cell lines, seeds of the iranian arnebia euchroma were collected from dena altitudes in the central zagross. All structured data from the file and property namespaces is available under the creative commons cc0 license. Mulching is preferred to reduce the moisture loss that helps germination enhancement. Stems are several, up to 40 cm tall, axillary, arising amidst the.
Their structures were determined by ms and nmr spectroscopy. Pdf arnebia euchroma rolye johnst boraginaceae and malva sylvestris l. The healing effect of arnebia euchroma ointment versus silver. Arnebia, a sub cosmopolitan and important genus of the boraginaceae family, comprises 25 species distributed among the world. The first and the last seeds germinated on the 4 thand 57 day of the experiment, respectively. All of the four arnebia polar extracts showed good radical scavenging activity with an averaged ic50 value of 3. Dec 10, 2015 arnebia euchroma exhibits a potent antihiv activity kashiwada et al.
Files are available under licenses specified on their description page. Standardization and antioxidant studies of arnebia hispidissima. Antimicrobial and antioxidant activity of methanol extracts. Antibacterial, antifungal and antioxidant activity of four. Bioassaydirected fractionation of extract of arnebia euchroma led to the isolation of alkannin 1, shikonin 2, and their derivatives 3. Antiinflammatory activity of shikonin derivatives from. Read anionic peroxidase production by arnebia euchroma callus, biotechnology and applied biochemistry on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Aug 02, 2016 avicennas canon of medicine, describing the use of abukhalsa arnebia euchroma ae as being effective for burn healing.
Secondary metabolites, arnebin 1, and arnebin 3 obtained from the other species of this genus are reported to possess anticancerous property harborne and baxter 1996. The aim of this research work was to evaluate the antimicrobial activity and antioxidant activity of methanolic plant extract. Arnebia euchroma in the germplasm resources information network grin, u. Pdf evaluation of burn healing properties of arnebia euchroma. The influence of light quality on the regeneration i. The antioxidant activities were evaluated using 2, 2diphenyl1picrylhydrazyl dpph free radical scavenging assays. National laboratory of drugs and devices for family planning, shanghai institute of planned parenthood re. Nov 01, 2011 arnebia euchroma callus, obtained from the root cell culture of an iranian native specimen, has gained a doubling time of 63 h after regular subculturing on linsmaierskoog ls medium containing sugar 50 gl, 2,4. Natural dyeing of wool with arnebia euchroma optimized by. Callus induction was successfully occurred for all collar, leaf and root explants on ms medium containing kinetin 10. The healing effect of arnebia euchroma ointment versus. Read antiinflammatory activity of shikonin derivatives from arnebia hispidissima, phytomedicine on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. The root of iranian arnebia euchroma produces shikalkin.
Chemical analysis showed that the dried root of the plant contained about 8. The homogenate extraction procedure was optimized and compared with other conventional extraction techniques. This article presents arnebia according to the most. Malvaceae are traditionally used to treat various skin disorders, and for antimicrobial. Standardization and antioxidant studies of arnebia. Arnebia euchroma royle johnston boraginaceae, a rich source of shikonin derivatives, is valued for its medicinal as well as coloring properties. Antibacterial, antifungal and antioxidant activity of four species from arnebia genus growing wild in iran a. Results callus induced from leaves of in vitro grown shoots of a. May 22, 2019 the influence of light quality on the regeneration i. Despite the observed somaclonal variations, peroxidase production by the a. Pdf pyrrolizidine alkaloids from arnebia euchroma researchgate. Arnebia euchroma wikispecies, free species directory.
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